Enhanced proliferative potential in culture of cells from p53-deficient mice

摘要: Normal somatic cells are endowed with limited doubling potential in culture, and the process of immortalization is an inevitable step neoplastic transformation cells. To examine roles p53 this process, p53-deficient mice were examined for potential. Fibroblast-like from a variety tissues these proliferated continuously without showing aging or crisis. The aneuploid overcome population passage, but cloning experiment indicated that chromosomal changes not essential to process. enhanced proliferative culture was also observed epithelial lens, mammary glands seminal vesicles neural precursor Proliferation bone marrow response stem cell factor long term vitro colony assay; no permanent lines could be obtained. No effects p53-deficiency found proliferation cardiac muscle hepatocytes.