Developmental Changes in the NAD Content in Sugar-Beet Root Mitochondria and Their Effect on the Oxidative Activity of These Organelles

摘要: The NAD content was determined in mitochondria isolated from sugar-beet roots at various stages of plant development. A high (7.6 ± 0.9 nmol/mg mitochondrial protein) observed the actively growing 80–95-day-old plants, but it decreased ca. twofold by end first year development, before were harvested for storage. stored low temperature two to three months and those after five eight days regrowth during second development manifested an even lower (2.2 0.4 2.0 0.5 protein, respectively). drastic decrease did not result impairment inner membrane these organelles evidently regulatory its nature. effect developmental changes intramitochondrial on malate oxidation pattern studied. In roots, limited rate state 3, because addition reaction mixture exogenous NAD, which can be transported matrix, promoted 30–50%. Rotenone inhibited stored-root more than 70%; this case, rotenone-resistant increased several times presence NAD. root, exo-genous affect oxidation, rotenone only 25–35%. analysis data obtained here published evidence suggests existence a universal mechanism respiration control regulation functional activity mitochondria. This acts through change organelle matrix. used course e.g., transition sugar-beet-root cells dormant state, when must decline.