Amyloid Fibrils form Hybrid Colloidal Gels and Aerogels with Dispersed CaCO3 Nanoparticles
作者： Yi Shen , Gustav Nyström , Raffaele Mezzenga
关键词: Scanning electron microscope 、 Crystallography 、 Diffusing-wave spectroscopy 、 Nanoparticle 、 Specific surface area 、 Chemical engineering 、 Fibril 、 Supercritical fluid 、 Self-healing hydrogels 、 Materials science 、 Colloid
摘要: New hybrid colloidal gels are reported formed by amyloid fibrils and CaCO3 nanoparticles (CaNPs), with Ca2+ as charge screening ions CaNPs physical crosslinking agents to establish stabilize the network. The gel is characterized rheological measurements diffusing wave spectroscopy, complemented microscopic observations using transmission scanning electron microscopy. show a two orders of magnitude improved strength at significantly shorter gelation times compared previous calcium ion-induced fibril gels. Supercritical CO2-dried aerogels allow demonstrating that fibrils, combined smaller (higher specific surface area) CaNPs, constitute denser network, resulting in stronger By varying concentration size concentration, complete phase diagram mapped out. This enables identifying sol–gel transition window for formation, which widens increasing size. Finally pH responsiveness self-healing properties this also demonstrated, making these systems suitable candidate biological applications.
handle.net参考文章(39)
Jin-Mi Jung, Raffaele Mezzenga, Liquid Crystalline Phase Behavior of Protein Fibers in Water: Experiments versus Theory Langmuir. ,vol. 26, pp. 504- 514 ,(2010) , 10.1021/LA9021432
J. Berger, M. Reist, J.M. Mayer, O. Felt, N.A. Peppas, R. Gurny, Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications European Journal of Pharmaceutics and Biopharmaceutics. ,vol. 57, pp. 19- 34 ,(2004) , 10.1016/S0939-6411(03)00161-9
Lingyun Chen, Gabriel E. Remondetto, Muriel Subirade, Food protein-based materials as nutraceutical delivery systems Trends in Food Science and Technology. ,vol. 17, pp. 272- 283 ,(2006) , 10.1016/J.TIFS.2005.12.011
Jozef Adamcik, Jin-Mi Jung, Jérôme Flakowski, Paolo De Los Rios, Giovanni Dietler, Raffaele Mezzenga, Understanding amyloid aggregation by statistical analysis of atomic force microscopy images Nature Nanotechnology. ,vol. 5, pp. 423- 428 ,(2010) , 10.1038/NNANO.2010.59
Thomas Billiet, Mieke Vandenhaute, Jorg Schelfhout, Sandra Van Vlierberghe, Peter Dubruel, A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering. Biomaterials. ,vol. 33, pp. 6020- 6041 ,(2012) , 10.1016/J.BIOMATERIALS.2012.04.050
Cecile Veerman, Hilde Ruis, Leonard M. C. Sagis, Erik van der Linden, Effect of electrostatic Interactions on the Percolation Concentration of Fibrillar ß-Lactoglobuline Gels Biomacromolecules. ,vol. 3, pp. 869- 873 ,(2002) , 10.1021/BM025533+
S. K. Maji, M. H. Perrin, M. R. Sawaya, S. Jessberger, K. Vadodaria, R. A. Rissman, P. S. Singru, K. P. R. Nilsson, R. Simon, D. Schubert, D. Eisenberg, J. Rivier, P. Sawchenko, W. Vale, R. Riek, Functional Amyloids as Natural Storage of Peptide Hormones in Pituitary Secretory Granules Science. ,vol. 325, pp. 328- 332 ,(2009) , 10.1126/SCIENCE.1173155
Nitin S. Satarkar, Dipti Biswal, J. Zach Hilt, Hydrogel nanocomposites: a review of applications as remote controlled biomaterials Soft Matter. ,vol. 6, pp. 2364- 2371 ,(2010) , 10.1039/B925218P
Dennis E Discher, Paul Janmey, Yu-li Wang, Tissue cells feel and respond to the stiffness of their substrate. Science. ,vol. 310, pp. 1139- 1143 ,(2005) , 10.1126/SCIENCE.1116995
Sreenath Bolisetty, Ludger Harnau, Jin-mi Jung, Raffaele Mezzenga, Gelation, Phase Behavior, and Dynamics of β-Lactoglobulin Amyloid Fibrils at Varying Concentrations and Ionic Strengths Biomacromolecules. ,vol. 13, pp. 3241- 3252 ,(2012) , 10.1021/BM301005W