Induction of dihydrolipoamide dehydrogenase in 3T3-L1 cells during differentiation.

摘要: The activity and turnover of dihydrolipoamide dehydrogenase (E3), the common component three 2-oxoacid complexes, were measured during differentiation 3T3-L1 preadipocytes into adipocytes. specific E3 increased approx. 3-4-fold in adipocytes differentiated under a regimen insulin, dexamethasone 3-isobutyl-1-methylxanthine for 48 h, followed by insulin alone thereafter. A rabbit antibody to pig heart quantitatively precipitated enzyme from By using immunoprecipitation gel electrophoresis, 3.3-fold increase was observed protein as compared with preadipocytes, on DNA basis. Pulse-labelling experiments L-[35S]methionine revealed 3.5-fold rate synthesis that preadipocytes. In contrast, apparent half-lives (43 h) (33 not significantly different. Therefore, resulted an enzyme.