Intertrubal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata
作者： Nguyen Duc Thanh , Anikó Páy , Marvin A. Smith , Péter Medgyesy , László Márton
DOI: 10.1007/BF00339580
关键词: Protoplast 、 Plastid 、 Nicotiana tabacum 、 Biology 、 Botany 、 Nicotiana 、 Chloroplast DNA 、 Salpiglossis 、 Salpiglossis sinuata 、 Chloroplast
摘要: Chloroplast tranfer was achieved by protoplast fusion between Nicotiana tobacum (Cestreae, Cestroideae) and Salpiglossis sinuata (Salpiglossideae, in the family Solanaceae. Isolation of cybrid clones facilitated irradiation cytoplasm donor protoplasts, use appropriate plastid mutants, streptomycin-resistant as donor, or light-sensitive recipient. Cybrid colonies were selected their green colour against background bleached (light-sensitive streptomycin-sensitive) colonies. In (Salpiglossis) plants possessing normal tobacco morphology chromsome number, presence Salpiglossis, plastids verified restriction analysis chloroplast DNA. A similar mitochondrial DNA these lines revealed unique, recombinant patterns case both fertile sterile plants. Progeny showed no appearance chlorophyll-deficiency F1 an additional back-cross generation. Attempts at transfer entire chloroplasts tabacum Solanum nigrum (Solaneae, Solanoideae) did not result any cell a medium suitable for colony formation species. These results suggest that fusion-mediated can surmount considerable taxonomical distance, but might be hampered plastome-genome incompatibility more remote combinations.
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