Extensive developmental and metabolic alterations in cybrids nicotiana tabacum (+ hyoscyamus niger) are caused by complex nucleo-cytoplasmic incompatibility

摘要: The genetic basis of multiple phenotypic alterations was studied in cell-engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome N. tabacum, plastome H. niger and recombinant mitochondria. plants possess a complex, maternally inheritable syndrome nucleo-cytoplasmic incompatibility, severely affecting growth, metabolism development. In vivo, manifested as: late germination seeds; dramatic decrease chlorophyll carotenoids cotyledons leaves; altered morphology cotyledons, leaves flowers; dwarfism. leaf phenotype depended on light intensity. 'green flowers' (an extreme phenotype), homeotic function B downregulated. vitro, incompatibility restricted to pigment deficiency cotyledons. Electron microscopy revealed perturbations differentiation chloroplasts palisade parenchyma cells bleached leaves. accompanied by retarded growth is discussed as result plastome–genome whereas other features are likely be due nucleo-mitochondrial incompatibilities.