Elongation of exogenous fatty acids by the bioluminescent bacterium Vibrio harveyi.

摘要: Bioluminescent bacteria require myristic acid (C14:0) to produce the myristaldehyde substrate of light-emitting luciferase reaction. Since both endogenous and exogenous C14:0 can be used for this purpose, metabolism fatty acids by luminescent has been investigated. Both Vibrio harveyi fischeri incorporated label from (1-14C)myristic into phospholipid acyl chains as well CO2. In contrast, Photobacterium phosphoreum did not exhibit acylation or beta-oxidation using acids. Unlike Escherichia coli, two species directly elongate such octanoic (C8:0), lauric (C12:0), acid, demonstrated radio-gas liquid chromatography. The induction bioluminescence in late exponential growth had little effect on ability V. acids, but it increase amount relative C16:0 labeled (14C)C8:0. This was observed a dark mutant that is incapable supplying luminescence. Cerulenin preferentially decreased labeling unsaturated all 14C-labeled precursors (14C)acetate, suggesting common mechanisms may involved elongation sources. Fatty acylationmore » luminescence-related synthetase reductase enzymes responsible aldehyde synthesis exhibited chain-length preference C14:0, which also indicated reverse-phase thin-layer chromatography groups attached these enzymes. activate related an adaptive requirement metabolize intracellular generated reaction during luminescence development.« less