Expression of Vibrio harveyi acyl-ACP synthetase allows efficient entry of exogenous fatty acids into the Escherichia coli fatty acid and lipid A synthetic pathways.

摘要: Although the Escherichia coli fatty acid synthesis (FAS) pathway is best studied type II system, a major experimental limitation has been inability to feed intermediates into in vivo because exogenously supplied free acids are not efficiently converted acyl-acyl carrier protein (ACP) thioesters required by pathway. We report that expression of Vibrio harveyi acyl-ACP synthetase (AasS), soluble cytosolic enzyme ligates ACP form acyl-ACPs, allows exogenous enter E. The incorporated intact and can be elongated or directly complex lipids acyltransferases specific for acyl-ACPs. Moreover, AasS strains supplementation with appropriate restored growth mutant lack essential enzymes. Thus, this strategy provides new tool circumventing loss enzymes FAS function.