A novel downstream process for 1,3-propanediol from glycerol-based fermentation

摘要: In this paper, a downstream process for purification of 1,3-propanediol from glycerol-based fermentation broth was investigated. The achieved by combining microfiltration, charcoal treatment, vacuum distillation, and silica gel chromatography. first filtered through hollow fiber cartridge, wherein 98.7% biomass removed. Soluble proteins other color impurities in the were removed use activated at optimal concentration 30 g l−1 where soluble decreased to 0.1 (96.0% protein loss). obtained when concentrated distillation resulted crystallization inorganic salts. Subsequently, purified gradient chromatography using as stationary phase mixture chloroform methanol mobile phase. Finally, with flow rate 10 ml min−1 loading amount 80 ml, yield 89%. overall proposed procedure 75.47%. developed method found be simple, rapid, efficient broth.