A systematic insight into a single-stage deammonification process operated in granular sludge reactor with high-loaded reject-water: characterization and quantification of microbiological community

摘要: Aims Reject-water from sludge dewatering was treated in a single-stage deammonification reactor. The aims were to characterize the microbiological community within granules. Methods and Results In situ mapping of intact granular made with fluorescent hybridization (FISH). Planctomycetes destroyed characterized by FISH, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), cloning sequence analysis. Conclusions AOB Betaproteobacteria concentrated first 50–100 μm granule, 100–200 μm responsible for nitrogen elimination. PCR-DGGE verified higher diversity reactor than anaerobic cultivation. analysis after identified dominant species anammox reaction as Ca. Brocadia fulgida (accession no. EU478693). FISH detection using universal probe AMX368 specific all bacteria including failed; however, BFU613 gave clear positive signals. three-dimensional structure ribosome may hinder binding corresponding 16S rRNA region. Significance Impact Study A combination multiple methods necessary. Oligonucleotide probes should be carefully selected, negative has other molecular biological techniques.