Detection of Erwinia amylovora in plant material using novel polymerase chain reaction (PCR) primers.

摘要: Abstract A rapid and sensitive method has been developed for the specific detection of Erwinia amylovora (Burr.) Winslow et al. using polymerase chain reaction (PCR). The involves amplification a 187 bp DNA fragment, probably chromosomal origin. All 69 cultures E. in an international collection from 10 host species five countries were successfully identified primers. In contrast, discrete PCR products not amplified 29 other or 20 plant pathogenic sapro‐phytic bacteria. detectable product was consistently reactions containing as few colony‐forming units culture tissue. field trials could detect apple flowers before fire blight symptoms occurred. This may have potential pre‐symptomatic disease management.