A Highly Conserved Vascular Permeability Factor Secreted by a Variety of Human and Rodent Tumor Cell Lines

摘要: We have previously reported that rodent tumor cell lines secrete a potent vascular permeability factor with molecular weight of 34,000-42,000 (Senger et al. Tumor cells promotes accumulation ascites fluid. Science (Wash. DC), 219: 983-985, 1983). This tumor-secreted (VPF) causes rapid and completely reversible increase in microvascular the species (guinea pig or rat) from which tumors were derived without causing mast degranulation endothelial damage exciting an inflammatory infiltrate. VPF may be responsible, at least part, for increased is commonly displayed by solid vessels. now examined 7 human determined 5 them also this same VPF. Antibody raised to guinea line 10 neutralized more than 90% permeability-increasing activity secreted these lines. Furthermore, VPFs both sources bound eluted similarly immobilized heparin comigrated identically on sodium dodecyl sulfate-polyacrylamide gels. Finally, 2 tumorigenic (in nude mice) found 14-fold their directly matched, nontumorigenic counterparts, suggesting elevated expression correlate neoplastic transformation. These data suggest broad spectrum several species, including humans, secretes highly conserved molecule enhances local function important growth.