Innervation of the male genital tract and kidney in the amphibia, Xenopus laevis Daudin, Rana temporaria L., and Bufo bufo L.

摘要: The innervation of the male genital tract and kidney in three anuran species was studied by fluorescence histochemical method Falck-Hillarp for demonstration monoamines whose identity secured thin-layer chromatography, electron microscopy including administration 5- or 6-hydroxydopamine (5- 6-OHDA). comprises testis, intra- extratesticular intrarenal seminal efferent ducts, Bidder's canal, renal dorsal transverse ureter. In addition-depending on studied-renal corpuscles various portions uriniferous tubules may be involved sperm transport. 1. Adrenaline is main transmitter nerves supplying kidney. Only Xenopus it possible to demonstrate presence noradrenaline, which confirmed Chromatographic analysis. No obvious changes are observed with regard distribution, amount, intensity adrenergic fibers their susceptibility towards 6-OHDA when comparing animals killed late autumn winter, spring, respectively. Non-adrenergic nerve have not been observed. 2. The testis only scarce confined blood vessels. Neuro-endocrine contacts Leydig cells established. gonadal ducts specific (i.e. non-vascular) intratesticular smooth muscle innervated. 3. Apart from (see below), ureter receives an which, however, even around time spermiation. Bundles nonterminal terminal axons seen running contiguous superficial bundles muscle-like cells. Neuromuscular relationships comprise synapses at distances 2000–5000 A, but no close contacts. vesicle Rana same mode apposition terminals observed. addition, a direct epithelium few instances. 4. In arteries, afferent arterioles, branches portal veins supplied dense plexus nerves. Small groups intensely fluorescent found walls proper. density arteriolar more pronounced Bufo than Xenopus. “ordinary” musculature membrane-to-membrane appositions, as well distance 800 4000n A juxtaglomerular epitheloid latter identified certainty. Adrenergic nerves, row glomeruli ventral muscular vein, reflect tubular because, that region, vessels do exist. Terminals partly completely devoid Schwann cell sheath approach basal lamina down 200 A. An related particular portion nephron observed; connecting also terminals.