Decompression induced bubble dynamics on ex vivo fat and muscle tissue surfaces with a new experimental set up.

摘要: Vascular gas bubbles are routinely observed after scuba dives using ultrasound imaging, however the precise formation mechanism and site of these still debated growth from decompression in vivo has not been extensively studied, due part to imaging difficulties. An experimental set-up was developed for optical recording bubble density on tissue surface area during hyperbaric decompression. Muscle fat tissues (rabbits, ex vivo) were covered with nitrogen saturated distilled water experiments performed, 3 0bar, at a rate 1bar/min. Pictures automatically acquired every 5s start 1h resolution 1.75μm. A custom MatLab analysis code implementing circular Hough transform written shown be able track sequences including center, radius, contact line angles over time. Bubble density, nucleation threshold detachment size, as well coalescence behavior, significantly different muscle surfaces, whereas rates critical size governed by diffusion expected. Heterogeneous preferential sites substrate, where grow, detach new form turn. No first 10min post so did vary this point experiment. In addition, competition dissolved between adjacent multiple demonstrated increased delay times slower non-isolated bubbles.