A single common electrophoretic abnormality of glucocorticoid receptors in human leukemia cells

摘要: We determined the mol wt of glucocorticoid receptors in human leukemia cells order to detect receptor defects that might cause resistance. Glucocorticoid intact were affinity labeled with [3H]dexamethasone-21-mesylate and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Receptors normal peripheral blood mononuclear six cell lines had 97,000. Malignant from ten 25 patients contained electrophoretically abnormal having 55,000 addition size (Mr = 97,000). The abnormality was not restricted a particular type seen both newly diagnosed who received prior chemotherapy, including prednisone. generated when only assayed under conditions favor proteolysis or cytosol containing form mixed receptors. same as mutant mouse lymphoma lines, S49 143R 55R, which have nuclear transfer-increased phenotype This work describes for first time single common electrophoretic cells. Further investigation could lead an improved understanding mechanisms resistance well method predicting are likely be resistant therapy.