Sample preparation procedures for biological atomic force microscopy.
作者： K. EL KIRAT , I. BURTON , V. DUPRES , Y. F. DUFRENE
DOI: 10.1111/J.1365-2818.2005.01480.X
关键词: Biomolecule 、 Microscopy 、 Nanotechnology 、 Protein crystallization 、 Atomic force microscopy 、 Biological sciences 、 Resolution (electron density) 、 Sample preparation 、 Materials science 、 Biological specimen
摘要: Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address structure, properties functions of specimens. AFM unique that provides three-dimensional images structures, including biomolecules, lipid films, 2D protein crystals cells, under physiological conditions with unprecedented resolution. A crucial prerequisite for successful, reliable samples need be well attached solid substrate using appropriate, nondestructive methods. In this review, we discuss common techniques immobilizing specimens studies.
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