Millimeter scale separation of DNA with a replaceable polymer matrix

作者: Dammika P. Manage , Duncan G. Elliott , Christopher J. Backhouse

DOI: 10.1002/ELPS.201200188

关键词: MillimeterNanotechnologyPolymerComputer scienceMatrix (chemical analysis)MicrofluidicsMicroelectronicsLab-on-a-chipPcr cloningScale separation

摘要: Electrophoresis is a powerful method that has seen wide range of applications, often in automated genetic diagnostic instruments require the use replaceable sieving matrix. The power and simplicity electrophoresis as an analysis technique would be ideal for highly integrated low-cost systems if could implemented microfluidics on scale several mm. We demonstrate electrophoretic DNA with separation lengths small 2 mm resolution adequate PCR products, i.e. resolutions 10-20 base pairs. Such small-scale separations enable consisting microelectronics into single inexpensive package, thereby overcoming key challenge facing development lab chip technologies.

参考文章(45)
Jon F. Merz, Antigone G. Kriss, Debra G. B. Leonard, Mildred K. Cho, Diagnostic testing fails the test Nature. ,vol. 415, pp. 577- 579 ,(2002) , 10.1038/415577A
Masanori Ueda, Yuriko Kiba, Hirohisa Abe, Akihiro Arai, Hiroaki Nakanishi, Yoshinobu Baba, Fast separation of oligonucleotide and triplet repeat DNA on a microfabricated capillary electrophoresis device and capillary electrophoresis. Electrophoresis. ,vol. 21, pp. 176- 180 ,(2000) , 10.1002/(SICI)1522-2683(20000101)21:1<176::AID-ELPS176>3.0.CO;2-A
Sundaresh N. Brahmasandra, Victor M. Ugaz, David T. Burke, Carlos H. Mastrangelo, Mark A. Burns, Electrophoresis in microfabricated devices using photopolymerized polyacrylamide gels and electrode-defined sample injection Electrophoresis. ,vol. 22, pp. 300- 311 ,(2002) , 10.1002/1522-2683(200101)22:2<300::AID-ELPS300>3.0.CO;2-F
Victor M. Ugaz, René D. Elms, Roger C. Lo, Faisal A. Shaikh, Mark A. Burns, Microfabricated electrophoresis systems for DNA sequencing and genotyping applications: current technology and future directions. Philosophical Transactions of the Royal Society A. ,vol. 362, pp. 1105- 1129 ,(2004) , 10.1098/RSTA.2003.1365
B. M. Paegel, C. A. Emrich, G. J. Wedemayer, J. R. Scherer, R. A. Mathies, High throughput DNA sequencing with a microfabricated 96-lane capillary array electrophoresis bioprocessor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 574- 579 ,(2002) , 10.1073/PNAS.012608699
Cheuk-Wai Kan, Christopher P. Fredlake, Erin A. S. Doherty, Annelise E. Barron, DNA sequencing and genotyping in miniaturized electrophoresis systems. Electrophoresis. ,vol. 25, pp. 3564- 3588 ,(2004) , 10.1002/ELPS.200406161
Chris Backhouse, Marcia Caamano, Frank Oaks, Eric Nordman, Albert Carrillo, Ben Johnson, Sue Bay, DNA sequencing in a monolithic microchannel device Electrophoresis. ,vol. 21, pp. 150- 156 ,(2000) , 10.1002/(SICI)1522-2683(20000101)21:1<150::AID-ELPS150>3.0.CO;2-5
Rafael Mateo, Ramón Gonzalez, Laura Sánchez-Hernández, Alejandro Cifuentes, Begoña Jiménez, Detection of Clostridium botulinum neurotoxin coding genes: analysis of PCR products by real time versus capillary gel electrophoresis methods European Food Research and Technology. ,vol. 227, pp. 495- 502 ,(2008) , 10.1007/S00217-007-0746-1