Controlled, Scalable Embryonic Stem Cell Differentiation Culture
作者： Stephen M. Dang , Sharon Gerecht-Nir , Jinny Chen , Joseph Itskovitz-Eldor , Peter W. Zandstra
DOI: 10.1634/STEMCELLS.22-3-275
关键词: Biology 、 KOSR 、 Cell biology 、 Stem cell 、 P19 cell 、 Cell potency 、 Cell aggregation 、 Embryoid body 、 Embryonic stem cell 、 Cellular differentiation 、 Developmental biology 、 Molecular medicine
摘要: Embryonic stem (ES) cells are of significant interest as a renewable source therapeutically useful cells. ES cell aggregation is important for both human and mouse embryoid body (EB) formation the subsequent generation derivatives. Aggregation between EBs (agglomeration), however, inhibits growth differentiation in stirred or high-cell-density static cultures. We demonstrate that agglomeration two initiated by E-cadherin-mediated attachment followed active migration. report development technology capable controlling cell-cell interactions scalable culture mass encapsulation size-specified agarose capsules. When placed stirred-suspension bioreactors, encapsulated can be used to produce quantities hematopoietic progenitor controlled environment.
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