Engineering vascularized skeletal muscle tissue.

摘要: One of the major obstacles in engineering thick, complex tissues such as muscle is need to vascularize tissue vitro. Vascularization vitro could maintain cell viability during growth, induce structural organization and promote vascularization upon implantation. Here we describe induction endothelial vessel networks engineered skeletal constructs using a three-dimensional multiculture system consisting myoblasts, embryonic fibroblasts cells coseeded on highly porous, biodegradable polymer scaffolds. Analysis conditions for stabilization vessels showed that addition increased levels vascular growth factor expression construct promoted formation vessels. We studied survival implants vivo three different models. Prevascularization improved vascularization, blood perfusion after transplantation.