New plasmid vectors for specific gene targeting in Spiroplasma citri

摘要: In Spiroplasma citri gene inactivation through homologous recombination has been achieved by using the replicative, oriC plasmid pBOT1 as disruption vector. However, required extensive passaging of transformants and, in most cases, occurred at rather than target gene. current study, we describe a new vector, which fragment was reduced to minimal sequences able promote replication. Using this vector inactivate motility scm1 showed that size reduction did increase frequency Furthermore, avoid transformants, developed strategy selective, tetracycline resistance phenotype can only be expressed once integrated into chromosome one single crossover As an example, targeting spiralin is described.