Enolase isoenzymes as markers of differentiation in teratocarcinoma cells and normal tissues of mouse

摘要: Abstract The changing profile of enolase (EC 4.2.1.11) isoenzymes in differentiating mouse cells has been traced by the use specific antisera to three subunits α, β, and γ. amounts were measured a variety tissues during normal development differentiation teratocarcinoma culture as tumors. One isoenzyme is predominant early developing embryo, namely, homodimer made up α subunits. same also sole form detected undifferentiated (embryonal carcinoma) cells. remains unchanged primitive definitive endoderm embryo. Similarly, formed embryonal carcinoma contained only αα enolase. In contrast, striated muscle brain, increasing proportions β γ subunits, respectively, detected. Thus was found be marker these tissues. This conclusion substantiated finding significant subunit cell cultures which had beating vitro .