Thermal control of droplets by nanoscale field effect transistors

摘要: Provided herein are methods and devices for rapidly accurately heating fluid droplets surrounded by a gas-phase medium, such as air. Sub-nanoliter can be heated nanoscale field effect transistors via microwave an applied AC voltage to the FET. The is in well-defined interior portion of droplet, with minimal outer thereby minimizing evaporation. In this manner, rapid thermal cycling possible, including independently parallel plurality droplets. Accordingly, provided used point-of-care detection PCR that high speed, robust low cost.