Cellular localization of a pollen-specific mRNA by in situ hybridization and confocal laser scanning microscopy

作者: W. H. Reijnen , M. M. A. van Herpen , P. F. M. de Groot , A. Olmedilla , J. A. M. Schrauwen

DOI: 10.1007/BF00200544

关键词: Cellular localizationBiophysicsIn situ hybridizationConfocal laser scanning microscopyPollen specificMolecular biologyMessenger RNAPollenBiology

摘要: The application of confocal laser scanning microscopy together with in situ hybridization experiments tobacco pollen enabled a detailed localization pollen-specific mRNA. three-dimensional distribution this specific mRNA over the whole grain was reconstructed by means optical sections one specimen.

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