作者: W. H. Reijnen , M. M. A. van Herpen , P. F. M. de Groot , A. Olmedilla , J. A. M. Schrauwen
DOI: 10.1007/BF00200544
关键词: Cellular localization 、 Biophysics 、 In situ hybridization 、 Confocal laser scanning microscopy 、 Pollen specific 、 Molecular biology 、 Messenger RNA 、 Pollen 、 Biology
摘要: The application of confocal laser scanning microscopy together with in situ hybridization experiments tobacco pollen enabled a detailed localization pollen-specific mRNA. three-dimensional distribution this specific mRNA over the whole grain was reconstructed by means optical sections one specimen.