Exosomal miR-21 promotes proliferation, invasion and therapy resistance of colon adenocarcinoma cells through its target PDCD4

摘要: Exosomes contain cell-specific collections of bioactive materials including proteins, lipids, and RNAs that are transported to recipient cells exert their impacts. MicroRNAs (miRNAs) can function as tumor suppressor or oncogenic genes miR-21 is one the most frequently up-regulated miRNAs in solid tumors colon cancer. The aim this study was investigate role miR-21, secreted from exosomes, proliferation invasion cancer, along with mechanistic details. We used a variety biochemical techniques ultracentrifugation-based exosome purification, electron transmission microscopy, western blot RT-qPCR detect expression levels exosomes purified culture media human colonic adenocarcinoma cell lines. then performed functional studies using three cancer lines HT29, T84 LS174 well normal epithelial CRL1831. target PDCD4 investigated for its mediating effects. Expression significantly cells, compared cells. Treatment isolated led an increased involved proliferation, extracellular matrix formation. targets PDCD4, TPM1 PTEN were down-regulated by silencing mimicked effects, even induced resistance against 5-FU. Our suggests targeted inhibition particularly those carrying may represent novel approach treatment colorectal