Ultrastructural demonstration of cell coat on the cell surfaces of normal human oesophageal epithelium

作者: Kathleen R. Logan , D. Hopwood , G. Milne

DOI: 10.1007/BF01002978

关键词: BiophysicsRuthenium redEpitheliumIntracellularCoatBiologyCellCell biologySafraninStainingUltrastructure

摘要: The cell coat in human oesophageal biopsies was studied with Alcian Blue, Ruthenium Red, Safranin O, colloidal iron and the ferrocyanide-osmium tetroxide techniques. Alcianophilic material found on surface of basal, prickle functional layers, being most abundant superficial cells where it appeared as a continuous coat. In deeper tended to have particulate distribution. Some membrane-coating granules were alcianophilic. Red had distribution over all surfaces. Intercellular debris also stained. O produced no staining. Colloidal stained manner. technique showed uniform filamentous epithelial coats are, part, acid mucosubstances which, cells, may protective function.

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