Ceratonia siliqua leaves exert a strong ROS-scavenging effect in human neutrophils, inhibit myeloperoxydase in vitro and protect against intestinal fluid and electrolytes secretion in rats

摘要: Chronic inflammation and oxidative stress are induced by biological, chemical physical factors which are, in turn, associated with an increased risk of several human diseases. Plants a large source new bioactive molecules therapeutic potential. In this respect, the present study was designed to investigate effects Ceratonia siliqua L. leaf aqueous extract (CSLAE) on neutrophil reactive oxygen species (ROS) production, vitro myeloperoxidase (MPO) activity expression as well small intestinal fluid electrolyte secretion. Neutrophils were isolated from whole blood healthy volunteers using ficoll–dextran method ROS generation H2O2 measured luminol amplified chemiluminescence. Superoxide anion also detected chemiluminescence lucigenin method. MPO quantity tetramethylbenzidine oxidation Western blotting analysis. Concerning vivo part, fasted male rats received gavage either vehicle (NaCl, 0.9%), at various doses (50, 100 200 mg kg−1) or clonidine (1 kg−1). An activated charcoal suspension administered oral gavage. Thirty minutes after receiving meal, euthanized intestine removed. The length distance traveled recorded. Castor oil-induced hypersecretion Wistar treated administration CSLAE antidiarrheal drug, Atropine (0.1 kg−1 i.p.). inhibited luminol-amplified (PMA)-stimulated neutrophils concentration-dependent manner is able scavenge superoxide anions hydrogen peroxide. reduces significantly dose-dependently expression. On other hand, studies showed that decreased notably GIT activity, concentration. analysis HPLC technique rich phenolic compounds, especially kaempferol, tannic acid catechin hydrate. intended inhibition introduced part strategy for pharmacological modulation chronic inflammatory processes. basis these findings, it can be assumed could potential novel discovery antidiarrhoeal drug development.