Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools

摘要: The silkworm, Bombyx mori, is a classic model organism in studies of insect genetics and physiology. As B. mori most other lepidopteran species (moths butterflies) are generally refractory to RNA interference (RNAi), it has been very difficult conduct loss-of-function genes lepidopterans. However, recent advances genome engineering tools, such as zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs), the clustered regularly interspaced palindromic repeats/CRISPR-associated (CRISPR/Cas) system, have dramatically changed this situation. Although efficiency targeted mutagenesis was low an early experiment using ZFNs, TALENs CRISPR/Cas9 induced highly efficient target even when mutant phenotype unknown. Genome editing tools facilitate sophisticated genetic manipulation breeding wide variety both beneficial pest insects. This chapter summarizes techniques proposes guidelines for experimental design strategy successful gene knockout experiments species.