Inhibition of the adherence of Escherichia coli strains to basement membrane by Lactobacillus crispatus expressing an S‐layer

作者: M. Horie , A. Ishiyama , Y. Fujihira-Ueki , J. Sillanpaa , T.K. Korhonen

DOI: 10.1046/J.1365-2672.2002.01539.X

关键词: Lactobacillus crispatusEnterobacteriaceaeEscherichia coliLactobacillusMicrobiologyBiologyLamininMatrigelBasement membraneMembrane glycoproteins

摘要: Aims: This study aimed to evaluate the efficiency with which Lactobacillus crispatus JCM 5810 inhibited adhesion of enteric pathogens a synthetic basement membrane and elucidate mechanism underlying inhibition. Methods Results: Lactobacillus three diarrhoeagenic Escherichia coli strains reconstituted preparation called Matrigel, used as model damaged intestinal tissue site. Inhibition was also observed use immobilized laminin, major component but diminished after removal S-layer protein (CbsA) from cells. The isolated CbsA E. both Matrigel laminin. seem inhibit pathogenic adhering via competition laminin molecules for binding sites. Conclusions: These results suggested that not only Lact. cells alone might prevent colonizing tissues. Significance Impact Study: This is first show applied aspect protein.

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