Indications that the erythrocyte receptor involved in enterotoxigenic Escherichia coli attachment is a sialoglycoconjugate.

摘要: A reverse hemagglutination assay was used to study adherence human erythrocytes by Escherichia coli H10407, which possesses colonization factor antigen I. Pretreatment of with trypsin, chymotrypsin, papain, protease, and neuraminidase completely abolished attachment reactivity. In addition, the reaction prevented presence urea guanidine. contrast, lipases, nucleotide hydrolases, exoglycosidases, reagents affecting disulfide or sulfhydryl moieties did not alter receptor Glycoconjugates containing sialic acid inhibited reaction. Furthermore, a sialoglycoprotein isolated from erythrocyte membrane Collectively, these data indicate that responsible for E. possessing I is sialoglycoconjugate.