Particulate tracers for homogeneous immunoassay - comprising antibody, antigen or hapten linked to particulate material without true light absorption

摘要: Particulate tracers for use in immunoassays comprise an immunoreactive substance (I) and a particulate label (II), is antibody, antigen or hapten; (II) particle with max. dimension of at least 0.2 micron comprising lyophobic amphilyophilic material having surface some (inherent induced) hydrophilicity without true absorption the visible/near-IR spectrum. The spectra their aggregates aq. medium are same, irrespective size attained by antibody-antigen agglutination reaction. Also claimed photometric immunoassay method determn. analyte, (a) attaching hapten, covalent coupling physical adsorption, to whose micron; (b) contacting resulting tracer analyte medium; (c) irradiating mixt. light wavelength 320-1000 nm; (d) measuring intensity transmitted reaction medium. USE/ADVANTAGE - may be used biological medical analysis, e.g. detection PCR amplification prods. (claimed) serum proteins, drugs such as gentamicin theophylline, pathogens Toxoplasma gondii. simple, uses stable reagents, gives limits ng/ml range good reproducibility.