Defocusing phase contrast effects in electron microscopy
作者： D. J. Johnson , D. Crawford
DOI: 10.1111/J.1365-2818.1973.TB03835.X
关键词: Transverse plane 、 Phase change 、 Confusion 、 Spherical aberration 、 Phase contrast microscopy 、 Optics 、 Contrast (vision) 、 Chemistry 、 Electron microscope 、 Pathology and Forensic Medicine 、 Histology
摘要: SUMMARY The theory of defocusing phase contrast is presented in terms Scherzer's (1949) ‘integrated-ray’ and Heidenreich's (1964) ‘single-ray’ approach to spherical aberration; transfer the Scherzer expression included. Fine structure transverse sections, negative stain carbon film shown be result contrast. Examples interpretative confusion are given for various macromolecular systems quantitative support found a simplified ‘rule-of-thumb’ phase-contrast enhancement. High-resolution studies carbon-fibre lattices demonstrate reversal, hence it possible show that practical change due aberration falls between Heidenreich terms; defocus term also require some adjustment.
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