Analysis of DNA damage in individual cells

摘要: Publisher Summary DNA is the most common intracellular target for action of agents used to treat malignant disease, and it an essential mutagenic agents. The comet or single-cell gel electrophoresis method can detect, either directly indirectly, a variety lesions, including single-strand breaks, doublestrand interstrand cross-links, damage bases. Apoptotic cells be easily identified because highly fragmented in apoptotic migrates efficiently. As few are required this method, fluorescence-activated cell sorting becomes practical way provide purified, populations analysis damage. Comets stained with antibodies against incorporated bromodeoxyuridine (BrdUrd) specific adducts. Much popularity assay due ability measure virtually any single cell. A more unique aspect detect heterogeneity response within different population. Under alkaline conditions, detects double-strand alkali labile lesions.