Control of tetrahydrobiopterin synthesis in T lymphocytes by synergistic action of interferon-gamma and interleukin-2.

摘要: The control of (6R)-5,6,7,8-tetrahydrobiopterin (H4biopterin) synthesis in primed T cells was analyzed by using the human cell leukemia virus type I (HTLV-I)-transformed line MT-2. In contrast to slowly progressing induction H4biopterin during activation resting cells, it is completed a 59-h period and directed synergism interferon-gamma (IFN-gamma) interleukin-2 (IL-2). Both GTP cyclohydrolase (6R)-(1‘,2‘-dioxopropyl)-5,6,7,8-tetrahydropterin synthase activities are induced IFN-gamma. They further enhanced combined treatment with IL-2, which per se ineffective. Furthermore, synchronizes time periods both maximum activities, now extending from 33 44 h. This correlates high cellular levels. It preceded fast transient increase depends on synergistic action IFN-gamma IL-2. coincides sepiapterin reductase activity. MT-2 HTLV-I-transformed HUT 102 constitutively secrete express mRNA. accumulation suppressed anti-IFN-gamma polyclonal antibody constitutive expression all H4 biopterin-synthesizing enzymes.