Ontogeny and aging of Nrf2 pathway genes in livers of rats.
作者： Shang-Fu Xu , Li-Li Ji , Qin Wu , Jin Li , Jie Liu
DOI: 10.1016/J.LFS.2018.04.018
关键词: Gene 、 Oxidative stress 、 Fetus 、 Endocrinology 、 Biology 、 Messenger RNA 、 GCLC 、 GCLM 、 Ontogeny 、 KEAP1 、 Internal medicine
摘要: Abstract The Nrf2/Keap1 antioxidant system plays important roles in protecting against oxidative stress and toxic stimuli, which may vary infants, elderly, females. Aim constitutive expression of the Nrf2 genes during development aging both sexes would help our understanding pathway toxicological studies. Main methods Sprague Dawley rat livers were collected at 11 age points from prenatal (−2 d), neonatal (1, 7, 14 21 puberty (28 35 adulthood (60 180 to (540 800 d) sexes. Total RNA proteins extracted for real-time RT-PCR Western-blot analysis. Key findings abundant mRNA was order Nrf2, Gclm, Nqo1, Gclc, Ho-1, Keap1. these except Gclc high fetal livers, decreased birth, reached first peak 7 days age, gradually adult levels till 180 days age. All remained 540 days but declined 800 days with more increases Nqo1 Ho-1. Females had lower fetal, neonatal, aged than males. Protein expressions GCLC GCLM agree Significance This study characterized age- sex-related changes Nrf2-related gene/proteins rats, higher newborns rats could cope increased infants elderly.
sci-hub.se 参考文章(54)
Ming-Liang Cheng, Yuan-Fu Lu, Hong Chen, Zhong-Yang Shen, Jie Liu, Liver expression of Nrf2-related genes in different liver diseases. Hepatobiliary & Pancreatic Diseases International. ,vol. 14, pp. 485- 491 ,(2015) , 10.1016/S1499-3872(15)60425-8
Runzhi Zhu, Yajing Wang, Liangqing Zhang, Qinglong Guo, Oxidative stress and liver disease Hepatology Research. ,vol. 42, pp. 741- 749 ,(2012) , 10.1111/J.1872-034X.2012.00996.X
Curtis D. Klaassen, Scott A. Reisman, Nrf2 the rescue: effects of the antioxidative/electrophilic response on the liver. Toxicology and Applied Pharmacology. ,vol. 244, pp. 57- 65 ,(2010) , 10.1016/J.TAAP.2010.01.013
Ping Jiang, Yuchen Sheng, Lili Ji, The age-related change of glutathione antioxidant system in mice liver. Toxicology Mechanisms and Methods. ,vol. 23, pp. 396- 401 ,(2013) , 10.3109/15376516.2013.769655
Rosario I. Bello, Consuelo Gómez-Díaz, María I. Burón, Francisco J. Alcaín, Plácido Navas, José M. Villalba, Enhanced anti-oxidant protection of liver membranes in long-lived rats fed on a coenzyme Q10-supplemented diet. Experimental Gerontology. ,vol. 40, pp. 694- 706 ,(2005) , 10.1016/J.EXGER.2005.07.003
Keiko Taguchi, Jonathan M. Maher, Takafumi Suzuki, Yukie Kawatani, Hozumi Motohashi, Masayuki Yamamoto, Genetic Analysis of Cytoprotective Functions Supported by Graded Expression of Keap1 Molecular and Cellular Biology. ,vol. 30, pp. 3016- 3026 ,(2010) , 10.1128/MCB.01591-09
Sergio Davinelli, Giovanni Scapagnini, Frank Denaro, Vittorio Calabrese, Francesca Benedetti, Selvi Krishnan, Sabrina Curreli, Joseph Bryant, Davide Zella, Altered expression pattern of Nrf2/HO-1 axis during accelerated-senescence in HIV-1 transgenic rat. Biogerontology. ,vol. 15, pp. 449- 461 ,(2014) , 10.1007/S10522-014-9511-6
Hongqiao Zhang, Honglei Liu, Kelvin J.A. Davies, Constantinos Sioutas, Caleb E. Finch, Todd E. Morgan, Henry Jay Forman, Nrf2-regulated phase II enzymes are induced by chronic ambient nanoparticle exposure in young mice with age-related impairments Free Radical Biology and Medicine. ,vol. 52, pp. 2038- 2046 ,(2012) , 10.1016/J.FREERADBIOMED.2012.02.042
Jonathan M. Davis, Richard L. Auten, Maturation of the antioxidant system and the effects on preterm birth. Seminars in Fetal & Neonatal Medicine. ,vol. 15, pp. 191- 195 ,(2010) , 10.1016/J.SINY.2010.04.001
Mateusz Kurzawski, Violetta Dziedziejko, Elżbieta Urasińska, Mariola Post, Maciej Wójcicki, Janusz Miętkiewski, Marek Droździk, Nuclear factor erythroid 2-like 2 (Nrf2) expression in end-stage liver disease. Environmental Toxicology and Pharmacology. ,vol. 34, pp. 87- 95 ,(2012) , 10.1016/J.ETAP.2012.03.001