Biosynthesis of Medium-Chain ω-Hydroxy Fatty Acids by AlkBGT of Pseudomonas putida GPo1 With Native FadL in Engineered Escherichia coli.

摘要: Hydroxy fatty acids (HFAs) are valuable compounds that widely used in medical, cosmetic and food fields. Production of ω-HFAs via bioconversion by engineered Escherichia coli has received lots attention because this process is environmentally friendly. In study, a whole-cell bio-catalysis strategy was established to synthesize medium-chain based on the AlkBGT hydroxylation system from Pseudomonas putida GPo1. The effects blocking β-oxidation (FAs) enhancing transportation FAs bio-production were also investigated. When fadE fadD deleted, consumption decanoic acid decreased, yield ω-hydroxydecanoic enhanced remarkably. Additionally, co-expression FA transporter protein, FadL, played an important role increasing conversion rate acid. As result, concentration NH03(pBGT-fadL) increased 309 mg/L 0.86 mol/mol, respectively. This further applied biosynthesis ω-hydroxyoctanoic ω-hydroxydodecanoic using octanoic dodecanoic as substrates, concentrations reached 275.48 249.03 mg/L, with yields 0.63 mol/mol 0.56 study demonstrated overexpression coupled native FadL efficient mutant E. strains.