A dual action of saturated fatty acids on electrical activity in rat pancreatic β-cells. Role of volume-regulated anion channel and KATP channel currents.

摘要: Non-technical summary  Saturated free fatty acids (FFAs) have been shown to exert initial stimulatory actions on insulin-producing cells, followed by a gradual impairment of insulin release. In this study we investigated the effects FFAs electrical properties rat insulin-secreting cells. We demonstrated an stimulation and prolonged inhibition activity FFAs, that could account for also explored underlying changes in ionic currents cells response FFAs. The results be relevant development metabolic abnormalities brought about high levels circulation. Abstract  Free complex pancreatic β-cells. Typically, potentiation release is β-cell function, latter effect being possible relevance hyperlipidaemia type 2 diabetes mellitus. molecular are poorly understood. present acute saturated electrophysiological responses Membrane potential KATP channel were recorded using perforated patch technique. Volume-regulated anion (VRAC) was assessed from conventional whole-cell recordings. Cell volume regulation measured video-imaging Addition octanoate caused transient glucose-induced activity, hyperpolarisation activity. Octanoate increase VRAC secondary coinciding with increased Similar observed palmitate 2-bromopalmitate whereas butyrate virtually ineffective. exerted dual evoked tolbutamide. significantly attenuated cell hypotonic solutions, consistent inhibition. It concluded medium long chain action β-cells: These appear result reciprocal currents, contribute towards inhibitory function.