Multivalent niacin nanoconjugates for delivery to cytoplasmic lipid droplets

摘要: We report here the design, synthesis, and properties, of multifunctional niacin nanoconjugates based on dendritic, miktoarm linear backbone nanocarriers, using “click” chemistry. The conjugates were in this instance used to deliver therapeutic agent lipid droplets. desired combination niacin, a lipophilic fluorescent dye (BODIPY), polyethylene glycol (PEG), was achieved by covalently linking agents selected carrier. nanocarriers containing BODIPY found almost exclusively within cytoplasmic droplets cells study (living hepatocytes microglia), whereas trifunctional carrier PEG partially localized these organelles but also elsewhere compartment. Spectrofluorometric analyses, confocal microscopy fluorescence cell sorting revealed different rates extent conjugate(s) internalization two types. Even micromolar concentrations internalized did not cause significant death or mitochondrial functional impairment, suggesting that they are suitable candidate nanostructures for droplet imaging targeting drugs cellular organelles. These studies provide an efficient easy way synthesize click chemistry, applicable synthesis related their use organelles, including