Application of PLGA/FGF-2 coaxial microfibers in spinal cord tissue engineering: an in vitro and in vivo investigation.
作者： Karina P Reis , Laura E Sperling , Cristian Teixeira , Ágata Paim , Bruno Alcântara
关键词: Biomedical engineering 、 Nestin 、 Spinal cord injury 、 Liberation 、 Systemic administration 、 Spinal cord 、 PLGA 、 Tissue engineering 、 In vivo 、 Chemistry
摘要: Aim Scaffolds are a promising approach for spinal cord injury (SCI) treatment. FGF-2 is involved in tissue repair but easily degradable and presents collateral effects systemic administration. In order to address the stability issue avoid effects, was encapsulated into core-shell microfibers by coaxial electrospinning its vitro vivo potential were studied. Materials & methods: The fibers characterized physicochemical biological parameters. scaffolds implanted hemisection SCI rat model. Locomotor test performed weekly 6 weeks. After this time, histological analyses expression of nestin GFAP quantified flow cytometry. Results: Electrospinning resulted uniform with structure, sustained liberation from fibers. supported PC12 cells adhesion proliferation. Implanted promoted locomotor recovery at 28 days after reduced expression. Conclusion These results indicate these engineering. [Formula: see text].
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