Sensitive determination method for mercury ion, methyl-, ethyl-, and phenyl-mercury in water and biological samples using high-performance liquid chromatography with chemiluminescence detection.
作者： Hitoshi KODAMATANI , Akito MATSUYAMA , Keiitsu SAITO , Yuriko KONO , Ryo KANZAKI
关键词: Chemiluminescence 、 Calibration curve 、 Chemistry 、 Certified reference materials 、 High-performance liquid chromatography 、 Reagent 、 Mercury (element) 、 Detection limit 、 Ethylmercury 、 Chromatography
摘要: A sensitive determination method for mercury speciation analysis was developed. Four species, ion, methylmercury, ethylmercury, and phenylmercury, were complexed with emetine-dithiocarbamate (emetine-CS(2)), then injected onto a HPLC instrument coupled tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection system. The emetine-CS(2) complexing agent effectively used to measure the concentration in addition serving as separation reagent. calibration curves these complexes linear range of 0.050 - 10 μg L(-1) (as Hg). limit (emetine-CS(2))(2)Hg, emetine-CS(2)-methylmercury, emetine-CS(2)-ethylmercury, emetine-CS(2)-phenylmercury 30, 17, 21, 22 ng L(-1), respectively. sensitivity this enables species water samples at sub-ppb levels. Furthermore, applied biological combination acid leaching liquid-liquid extraction using an results good agreement values certified reference materials.
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