Electrogenerated Chemiluminescence Derivatization Reagent, 3-Isobutyl-9,10-dimethoxy-1,3,4,6,7,11b- hexahydro-2H-pyrido[2,1-a]isoquinolin-2-ylamine, for Carboxylic Acid in High-Performance Liquid Chromatography Using Tris(2,2‘-bipyridine)ruthenium(II)

摘要: 3-Isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoquinolin-2-ylamine (IDHPIA) was found to be a selective and highly sensitive derivatization reagent for carboxylic acid by high-performance liquid chromatography (HPLC) with electrogenerated chemiluminescence detection using tris(2,2‘-bipyridine)ruthenium(II). Free fatty acids phenylbutylic were used as model compounds of acids, the conditions optimized myristic acid. Under mild reaction room temperature 45 min in acetonitrile containing 2-bromo-1-ethylpyridinium tetrafluoroborate 9-methyl-3,4-dihydro-2H-pyrido[1,2-a]pyrimidin-2-one, all tested reacted IDHPIA produce derivatives. The intensity essentially same acids. derivatives obtained from 10 free completely separated reversed-phase under isocratic elution conditions. on-column detect...