Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves.

摘要: Triacylglycerol (TAG) is an energy-rich reserve in plant seeds that composed of glycerol esters with three fatty acids. Since TAG can be used as a feedstock for the production biofuels and bio-chemicals, producing TAGs vegetative tissue alternative way meeting increasing demand its usage. The WRINKLED1 (WRI1) gene well-established key transcriptional regulator involved upregulation acid biosynthesis developing seeds. WRI1s from Arabidopsis several other crops have been previously employed seed tissues. In present study, we first identified functional CsWRI1 genes (CsWRI1A. B, C) Camelina oil crop tested their ability to induce synthesis leaves. amino sequences CsWRI1s exhibited more than 90% identity those WRI1. transcript levels showed higher expression floral When CsWRI1A. or C was introduced into wri1-3 loss-of-function mutant, content restored near wild-type percentages wrinkled were remarkably reduced transgenic lines relative mutant line. addition, fluorescent signals enhanced yellow protein (eYFP) fused observed nuclei Nicotiana benthamiana leaf epidermal cells. Nile red staining indicated transient caused accumulation bodies N. by approximately 2.5- 4.0-fold fresh leaves expressing control These results suggest regulators increase acids biomass.