Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
作者： Aruna D Wickramarathna , Rodrigo M P Siloto , Elzbieta Mietkiewska , Stacy D Singer , Xue Pan
DOI: 10.1186/S12896-015-0156-6
关键词: Biochemistry 、 Amino acid 、 Arabidopsis thaliana 、 Diacylglycerol cholinephosphotransferase 、 Biology 、 Diacylglycerol kinase 、 Linum 、 Polyunsaturated fatty acid 、 Complementation 、 Oleic acid
摘要: … In this study, we describe the isolation and characterization of two PDCT genes from flax (LuPDCT1 and LuPDCT2) with very high nucleotide sequence identity (97%) whose deduced …
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W Stoffel, K Hofmann, Tmbase-A database of membrane spanning protein segments Biol. Chem. Hoppe-Seyler. ,vol. 374, pp. 166- 166 ,(1993)
Jane Glazebrook, Detlef Weigel, Arabidopsis : a laboratory manual Weigel, Detlef , Glazebrook, Jane Arabidopsis. pp. 1354- ,(2002)
J E Stukey, V M McDonough, C E Martin, Isolation and characterization of OLE1, a gene affecting fatty acid desaturation from Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 264, pp. 16537- 16544 ,(1989) , 10.1016/S0021-9258(19)84740-3
Philip D. Bates, John Browse, The pathway of triacylglycerol synthesis through phosphatidylcholine in Arabidopsis produces a bottleneck for the accumulation of unusual fatty acids in transgenic seeds Plant Journal. ,vol. 68, pp. 387- 399 ,(2011) , 10.1111/J.1365-313X.2011.04693.X
S.C. Morash, C.R. McMaster, R.H. Hjelmstad, R.M. Bell, Studies employing Saccharomyces cerevisiae cpt1 and ept1 null mutants implicate the CPT1 gene in coordinate regulation of phospholipid biosynthesis. Journal of Biological Chemistry. ,vol. 269, pp. 28769- 28776 ,(1994) , 10.1016/S0021-9258(19)61972-1
Roger P. Hellens, E. Anne Edwards, Nicola R. Leyland, Samantha Bean, Philip M. Mullineaux, pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology. ,vol. 42, pp. 819- 832 ,(2000) , 10.1023/A:1006496308160
Maren Rossak, Mark Smith, Ljerka Kunst, Expression of the FAE1 gene and FAE1 promoter activity in developing seeds of Arabidopsis thaliana. Plant Molecular Biology. ,vol. 46, pp. 717- 725 ,(2001) , 10.1023/A:1011603923889
Elzbieta Mietkiewska, Rodrigo M. P. Siloto, Jay Dewald, Saleh Shah, David N. Brindley, Randall J. Weselake, Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1Δ mutant strain of Saccharomyces cerevisiae. FEBS Journal. ,vol. 278, pp. 764- 775 ,(2011) , 10.1111/J.1742-4658.2010.07995.X
Junghoon Lee, Ruth Welti, William T. Schapaugh, Harold N. Trick, Phospholipid and triacylglycerol profiles modified by PLD suppression in soybean seed Plant Biotechnology Journal. ,vol. 9, pp. 359- 372 ,(2011) , 10.1111/J.1467-7652.2010.00562.X
KK Singh, D Mridula, Jagbir Rehal, P Barnwal, None, Flaxseed: a potential source of food, feed and fiber. Critical Reviews in Food Science and Nutrition. ,vol. 51, pp. 210- 222 ,(2011) , 10.1080/10408390903537241