The Role of Antigen-Presenting Cells in the Regulation of Delayed-Type Hypersensitivity

摘要: Abstract A new mechanism for regulation of delayed-type hypersensitivity (DTH) was investigated. The subcutaneous injection without adjuvant syngeneic epidermal Langerhans' cells (LC) pulsed with keyhole limpet hemocyanin (KLH) into BALB/c mice gave rise to DTH upon challenge the ear same antigen. When such were transferred intravenously, did not occur, although titer anti-KLH antibodies high. Peritoneal exudate macrophages (Mo) KLH neither nor antibody production. intravenous transfer KLH-pulsed LC immunized subcutaneously in complete Freund's at time (in sensitization phase) had a suppressive effect on an H-2-restricted way. Mo have immunoregulatory effects. radiolabeled and they migrated spleen, but when subcutaneously, stayed skin or lymph nodes. In splenectomized KLH, caused production suppressed DTH. Ia expressed surface Mo, could present antigens, as could. These findings suggest that anatomic sites which antigen is presented (i.e., spleen draining nodes) rather than kind cell first presents immune system important deciding whether response takes place