Simultaneous Evaluation of Cellular Vitality and Drug Penetration in Multicellular Layers of Human Cancer Cells
作者：
DOI: 10.4333/KPS.2006.36.5.309
关键词: Penetration (firestop) 、 Biophysics 、 Human cancer 、 Colorectal cancer 、 Cancer cell 、 Nanotechnology 、 Confocal imaging 、 Drug 、 Chemistry 、 Drug penetration 、 Multicellular organism
摘要: The multicellular layers(MCL) of human cancer cells is a three dimensional(3D) in vitro model for solid tumors which has been used primarily the assessment avascular penetration anti-cancer drugs. For drugs with problem, MCL represents good experimental that can provide clinically relevant data. Calcein-AM fluorescent dye demonstrates cellular vitality graded manner cell culture system. In present study, we evaluated use calcein-AM determination anti-proliferative activity agents DLD-1 colorectal cells. Optical sectioning confocal imaging was compromised photonic attenuation and barrier deep layers MCL. By contrast, measurement on cryo-sections provided feasible alternative. Cold pre-incubation did not enhance distribution to significant degree However, simultaneous drug appeared be possible treated conclusion, these data suggest drug-induced effect model.
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