Studies on fatty liver with isolated hepatocytes
作者： Giuseppe Poli , Enrico Gravela , Emanuele Albano , Mario U. Dianzani
DOI: 10.1016/0014-4800(79)90086-8
关键词: Lipoprotein 、 Fatty liver 、 Carbon tetrachloride 、 Lipid peroxidation 、 In vivo 、 Secretion 、 Metabolism 、 Secretory pathway 、 Chemistry 、 Biochemistry 、 Pathology and Forensic Medicine 、 Clinical biochemistry 、 Molecular biology
摘要: Abstract This report describes some effects of poisoning with carbon tetrachloride on hepatocytes in single cell suspension. In isolated liver cells as well “ vivo ” CCl 4 stimulates lipid peroxidation, inhibits both protein synthesis and lipoprotein secretion induces fat accumulation within the cells. As action our data confirm that its increase induced by depends metabolism this drug NADPH-cytochrome P 450 enzyme system. Furthermore, reported here suggest onset -induced decrease is due to a derangement secretory pathway.
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sci-hub.se 参考文章(26)
K N Jeejeebhoy, J Ho, G R Greenberg, M J Phillips, A Bruce-Robertson, U Sodtke, Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis. Biochemical Journal. ,vol. 146, pp. 141- 155 ,(1975) , 10.1042/BJ1460141
C C Weddle, K R Hornbrook, P B McCay, Lipid peroxidation and alteration of membrane lipids in isolated hepatocytes exposed to carbon tetrachloride. Journal of Biological Chemistry. ,vol. 251, pp. 4973- 4978 ,(1976) , 10.1016/S0021-9258(17)33209-X
Frederick Bernheim, Mary L.C. Bernheim, Karl M. Wilbur, The reaction between thiobarbituric acid and the oxidation products of certain lipides. Journal of Biological Chemistry. ,vol. 174, pp. 257- 264 ,(1948) , 10.1016/S0021-9258(18)57394-4
M. U. Dianzani, E. Gravela, Inhibition of protein synthesis in carbon tetrachloride-induced liver injury Springer, Dordrecht. pp. 225- 238 ,(1975) , 10.1007/978-94-011-6618-8_20
Enrico Gravela, Giuseppe Poli, Emanuele Albano, Mario U. Dianzani, Studies on fatty liver with isolated hepatocytes Experimental and Molecular Pathology. ,vol. 27, pp. 339- 352 ,(1977) , 10.1016/0014-4800(77)90005-3
Per O. Seglen, Preparation of isolated rat liver cells. Methods in Cell Biology. ,vol. 13, pp. 29- 83 ,(1976) , 10.1016/S0091-679X(08)61797-5
Yannick Le Marchand, Amreek Singh, Françoise Assimacopoulos-Jeannet, Lelio Orci, Charles Rouiller, Bernard Jeanrenaud, A Role for the Microtubular System in the Release of Very Low Density Lipoproteins by Perfused Mouse Livers Journal of Biological Chemistry. ,vol. 248, pp. 6862- 6870 ,(1973) , 10.1016/S0021-9258(19)43430-3
Trevor Frank Slater, Free radical mechanisms in tissue injury ,(1972)
K. Suryanarayana Rao, Richard O. Recknagel, Early incorporation of carbon-labeled carbon tetrachloride into rat liver particulate lipids and proteins Experimental and Molecular Pathology. ,vol. 10, pp. 219- 228 ,(1969) , 10.1016/0014-4800(69)90041-0
E. Gravela, M.U. Dianzani, Studies on the mechanism of CCl4
-induced polyribosomal damage FEBS Letters. ,vol. 9, pp. 93- 96 ,(1970) , 10.1016/0014-5793(70)80321-0