The effect of different methanol concentrations and temperatures on the extraction of mycosporine-like amino acids (MAAs) in algae and zooplankton

摘要: Mycosporine-like amino acids (MAAs) are a family of intracellular UV-absorbing compounds involved in the protection aquatic organisms against solar UV radiation. These have been identified and quantified many marine freshwater organisms, however, no standard method to analyze these is available. Consequently, protocols extract MAAs largely differ, for example, type solvent, concentrations, temperatures, times used. In this study, we tested whether concentrations affected by extraction at different temperatures methanol (MeOH) concentrations. Natural assemblages phytoplankton, cyclopoid copepod Cyclops abyssorum tatricus, alga Porphyra sp. were used as test organisms. The shinorine, palythine, MAA 331, asterina-330, porphyra-334 present examined generally best extracted 45 °C 25 % aqueous MeOH. natural mean total obtained with protocol respectively -13 ∼3 higher than when 100% MeOH 4 °C. Cyclops, also highest °C, but there was statistically significant difference among (P = 0.079). Depending on organism examined, both concentration temperature efficiency final concentration. Our results stress need priori testing influence variables assure that without altering qualitative composition. Based results, direct comparison reported scientific literature should be done caution.