Efficient synthesis of vitamin E intermediate by lipase-catalyzed regioselective transesterification

摘要: Abstract Trimethylhydroquinone-1-monoacetate (TMHQ-1-MA) is a valuable synthetic intermediate for vitamin E acetate. Immobilized Lipozyme RM IM from Mucor miehei was shown to be the best biocatalyst production of TMHQ-1-MA through regioselective transesterification between trimethylhydroquinone diacetate (TMHQ-DA) and alcohol. The effects lipase-catalyzed reaction conditions including solvent, acyl receptor, substrate mole ratio, temperature agitation speed were investigated. optimum catalyzed achieved at ratio 1:1, an 200 rpm 50 °C in MTBE/ n -hexane (3:7). Under above conditions, exhibited high tolerance (substrate concentrations 1.06 M). Recycling experiments demonstrated that quite steady under conditions. analysis kinetic experiment showed enzymatic obeys Ping–Pong bi–bi mechanism with -butanol inhibition.