Protein modifications by electrophilic lipoxidation products: adduct formation, chemical strategies and tandem mass spectrometry for their detection and identification.

摘要: The post-translational modification of proteins by electrophilic oxylipids is emerging as an important mechanism that contributes to the complexity proteomes. Enzymatic and non-enzymatic oxidation biological lipids results in formation chemically diverse carbonyl compounds, such 2-alkenals 4-hydroxy alkenals, epoxides, eicosanoids with reactive cyclopentenone structures. These lipoxidation products are capable modifying proteins. Originally considered solely markers oxidative insult, more recently modifications lipid peroxidation being recognized a new cell signaling relevance redox homeostasis, adaptive response inflammatory resolution. growing interest protein oxylipid species necessitates availability methods detecting, identifying characterizing these adducts samples high complexity. However, efficient analysis presents considerable analytical challenge. We first provide introduction into chemistry adductions products. then overview tandem mass spectrometry approaches have been developed recent years for interrogation species.