Cell culture chip using low-shear mass transport.
作者： Ke Liu , Rajasekar Pitchimani , Dana Dang , Keith Bayer , Tyler Harrington
DOI: 10.1021/LA8003917
关键词: Microfluidics 、 Composite material 、 Shear stress 、 Materials science 、 Cell culture 、 Shear force 、 Volumetric flow rate 、 Microscopy 、 Mineralogy 、 Shear (sheet metal) 、 Suspension (vehicle)
摘要: We have developed a flow cell that allows culturing adherent cells as well suspended in stable, homogeneous, and low-shear force environment. The device features continuous medium supply waste exchange. In this paper, simple fast protocol for design, fabrication, assembly (sealing) based on poly(dimethylsiloxane) (PMDS)/glass slide hybrid structure is described. culture system performance was monitored, the effective shear inside also determined. By manipulating dimensions volumetric rate, stress controlled during experiments. Cell adhesion, growth, proliferation, death over long-term periods were observed by microscopy. growth of both endothelial suspension exhibited comparable characteristics to those traditional approaches. significantly reduced encountered microfluidic systems, allowing be grown device.
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