The Binding of Steroid Hormones to Degranulated Microsomes from Rat‐Liver Endoplasmic Reticulum

作者: Carol A. Blyth , Michael B. Cooper , Anne Roobol , Brian R. Rabin

DOI: 10.1111/J.1432-1033.1972.TB01988.X

关键词: BiologyBiochemistryMembraneMicrosomeBinding siteEndoplasmic reticulumInternal medicineTestosteronePolysomeEndocrinologyCell fractionationSteroid

摘要: 1 Smooth reticular membranes from male rat liver contain high affinity binding sites for oestradiol. Similar females analogous testosterone. 2 Rough which polysomes have been removed by EDTA (degranulated membranes), no either hormone. 3 Ethyl acetate extracts of degranulated materials block the on smooth same but not opposite sex. 4 These promote attachment ribosomes to sex. 5 The ethyl and female substances behave like oestradiol testosterone, respectively.

参考文章(8)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Gerhard Schmidt, S.J. Thannhauser, A METHOD FOR THE DETERMINATION OF DESOXYRIBONUCLEIC ACID, RIBONUCLEIC ACID, AND PHOSPHOPROTEINS IN ANIMAL TISSUES Journal of Biological Chemistry. ,vol. 161, pp. 83- 89 ,(1945) , 10.1016/S0021-9258(17)41524-9
R.B. Heap, A.M. Symons, J.C. Watkins, Steroids and their interactions with phospholipids: Solubility, distribution coefficient and effect on potassium permeability of liposomes Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 218, pp. 482- 495 ,(1970) , 10.1016/0005-2760(70)90011-1
CAROL A. BLYTH, R. B. FREEDMAN, B. R. RABIN, Sex Specific Binding of Steroid Hormones to Microsomal Membranes of Rat Liver Nature. ,vol. 230, pp. 137- 139 ,(1971) , 10.1038/NEWBIO230137A0
G. H. SUNSHINE, D. J. WILLIAMS, B. R. RABIN, Role for steroid hormones in the interaction of ribosomes with the endoplasmic membranes of rat liver. Nature. ,vol. 230, pp. 133- 136 ,(1971) , 10.1038/NEWBIO230133A0
Carol A. Blyth, Robert B. Freedman, Brian R. Rabin, The effects of aflatoxin B1 on the sex-specific binding of steroid hormones to microsomal membranes of rat liver. FEBS Journal. ,vol. 20, pp. 580- 586 ,(1971) , 10.1111/J.1432-1033.1971.TB01430.X
A. Fleck, D. Begg, The estimation of ribonucleic acid using ultraviolet absorption measurements Biochimica et Biophysica Acta. ,vol. 108, pp. 333- 339 ,(1965) , 10.1016/0005-2787(65)90025-0